AIMS Geonetwork
Sensitive environmental DNA detection via lateral flow assay (dipstick) - A case study on corallivorous crown-of-thorns sea star (Acanthaster cf. solaris) detection
Detection of low-density CoTS populations is critical for early warning of outbreaks and can lead to early management interventions (e.g., culling). A lateral flow assay method for eDNA detection was developed using a commercially available nucleic acid lateral flow device (PCRD™) in combination with a previously developed species-specific mtDNA primer. This method was applied to eDNA water samples collected in field locations where CoTS were present in low (=nonoutbreak) population densities to demonstrate the sensitivity of the method to detect these populations.
Aquarium raised 6- to 12-month-old juvenile CoTS were used for laboratory testing to prepare dilution series of genomic DNA.
A total of 58 water samples were collected from two locations on the Great Barrier Reef (GBR), Australia; 40 samples from Big Vicky's Reef in August 2019 and 18 samples from Elizabeth Reef in May 2019. See Doyle and Uthicke (2021) for full sample processing procedures.
Each field sample was analysed via ddPCR and PCR/LFA and assay sensitivity was compared as well as an assessment of the cost and time to complete sample analysis.
All data associated with this study can be found either in the main text or appendices of Doyle and Uthicke (2021) linked below.
Simple
Identification info
- Date (Revision)
- 2024-10-17T00:00:00
Publisher
Owner
- Website
- AIMS Web Site
- Website
- AIMS Web Site
- Credit
- Uthicke, S. (AIMS)
- Credit
- Doyle, J. Australian Institute of Marine Science (AIMS)
- Status
- Completed
Principal investigator
Point of contact
- Temporal resolution
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P1Y0M0DT0H0M0S
- Topic category
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- Oceans
Extent
- Description
- Elizabeth Reef
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Extent
- Description
- Big Vicky's Reef, Lizard Island
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Extent
- Description
- Collective resources start and end dates
Temporal extent
- Time position
- 2019-05-17
- Time position
- 2019-08-29
- Maintenance and update frequency
- As needed
- AODN Discovery Parameter Vocabulary
Resource constraints
- Linkage
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http://i.creativecommons.org/l/by/3.0/au/88x31.png
License Graphic
- Title
- Creative Commons Attribution 3.0 Australia License
- Website
-
http://creativecommons.org/licenses/by/3.0/au/
License Text
- Other constraints
- Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.
- Other constraints
- Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2021). Sensitive environmental DNA detection via lateral flow assay (dipstick) - A case study on corallivorous crown-of-thorns sea star (Acanthaster cf. solaris) detection. https://apps.aims.gov.au/metadata/view/e19d6487-3283-46e3-b0da-1185b66d9e97, accessed[date-of-access]".
- Language
- English
- Character encoding
- UTF8
Content Information
- Content type
- Physical measurement
Identifier
- Code
- Abundance of biota
- Description
- The relative representation of flora and fauna in a particular sample or region.
Distribution Information
Distributor
Distributor
- OnLine resource
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Doyle, J, Uthicke, S. Sensitive environmental DNA detection via lateral flow assay (dipstick)—A case study on corallivorous crown-of-thorns sea star (Acanthaster cf. solaris) detection. Environmental DNA. 2021; 3: 323– 342. https://doi.org/10.1002/edn3.123
Doyle, J, Uthicke, S. Sensitive environmental DNA detection via lateral flow assay (dipstick)—A case study on corallivorous crown-of-thorns sea star (Acanthaster cf. solaris) detection. Environmental DNA. 2021; 3: 323– 342. https://doi.org/10.1002/edn3.123
Resource lineage
- Hierarchy level
- Dataset
- Maintenance and update frequency
- As needed
Metadata
- Metadata identifier
- e19d6487-3283-46e3-b0da-1185b66d9e97
- Language
- English
- Character encoding
- UTF8
Point of contact
- Hours of service
- 0800 to 1640 UTC+10: Monday to Friday
Type of resource
- Resource scope
- Dataset
- Metadata linkage
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Point of truth URL of this metadata record
Point of truth URL of this metadata record
- Date info (Creation)
- 2021-11-03T00:00:00
- Date info (Revision)
- 2021-11-08T00:00:00
Metadata standard
- Title
- ISO 19115-3:2018
Overviews
Spatial extent
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