AIMS Geonetwork
Uptake of dissolved free amino acids by larvae of the crown-of-thorns starfish, Acanthaster planci, from the Great Barrier Reef
Early brachiolaria larvae (6 days old) were reared at the Australian Institute of Marine Science for use in dissolved organic matter uptake experiments. Three dissolved free amino acids (substrates) were chosen for the experiments: alanine (neutral); glutamic acid (acidic); and arginine (basic). Between 120 and 240 larvae were placed in individual scintillation vials containing filtered seawater and maintained at 29.5°C. Experiments were run using filtered seawater with only one substrate. Different amounts of substrates were added to vials with or with larvae, as well as vials with formalin killed larvae. Triplicate 500 µl samples were withdrawn from each vial initially and then after incubation for 20, 40 and 60 minutes. Samples were passed through a 100 µm mesh screen to remove larvae and frozen in a micro-centrifuge tube for later DFAA analysis using the pre-column derivatization method with HPLC.
This research was undertaken to investigate the ability of early brachiolaria larvae of the crown-of-thorns starfish, Acanthaster planci, to take up dissolved organic matter (DOM) in the form of dissolved free amino acids (DFAA).
Simple
Identification info
- Date (Revision)
- 2024-10-17T00:00:00
Publisher
Owner
- Website
- AIMS Web Site
- Website
- AIMS Web Site
- Credit
- Okaji, Ken, Dr (Principal Investigator)
- Status
- Completed
Principal investigator
Point of contact
- Temporal resolution
-
P1Y0M0DT0H0M0S
- Topic category
-
- Oceans
Extent
- Description
- Region 1
))
Extent
- Description
- Collective resources start and end dates
Temporal extent
- Time position
- 1991-01-01
- Time position
- 1993-12-31
- Maintenance and update frequency
- Not planned
Resource constraints
- Linkage
-
http://i.creativecommons.org/l/by-nc/3.0/au/88x31.png
License Graphic
- Title
- Creative Commons Attribution-NonCommercial 3.0 Australia License
- Website
-
http://creativecommons.org/licenses/by-nc/3.0/au/
License Text
- Other constraints
- Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.
- Other constraints
- Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2010). Uptake of dissolved free amino acids by larvae of the crown-of-thorns starfish, Acanthaster planci, from the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/41bda75c-97d3-48a1-ace3-96159c97e2eb, accessed[date-of-access]".
- Other constraints
- Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.
- Language
- English
- Character encoding
- UTF8
Content Information
- Content type
- Physical measurement
Distribution Information
Distributor
Distributor
- OnLine resource
-
Selective uptake of dissolved free amino acids by the larvae of the crown-of-thorns starfish, Acanthaster planci (L.): Okaji K, Ayukai T and Lucas JS (1997) Selective uptake of dissolved free amino acids by the larvae of the crown-of-thorns starfish, Acanthaster planci (L.). 1: 613-616. In: Proceedings of the 8th International Coral Reef Symposium, Panama, 24-29 June 1996. Smithsonian Tropical Research Institute.
Selective uptake of dissolved free amino acids by the larvae of the crown-of-thorns starfish, Acanthaster planci (L.): Okaji K, Ayukai T and Lucas JS (1997) Selective uptake of dissolved free amino acids by the larvae of the crown-of-thorns starfish, Acanthaster planci (L.). 1: 613-616. In: Proceedings of the 8th International Coral Reef Symposium, Panama, 24-29 June 1996. Smithsonian Tropical Research Institute.
Resource lineage
- Statement
- Statement: The techniques used for the rearing of larval crown-of-thorns starfish are described in: Ayukai T, Keesing J, Cartwright C, Halford A, Hall K, Hocking D and Okaji K (1996) Practical handbook for large scale rearing of larvae and juveniles of the crown-of-thorns starfish. Australian Institute of Marine Science, Townsville, 25p.The methods used for DFAA analysis are described in:Mopper K and Lindroth P (1982) Diel and depth variations in dissolved free amino acids and ammonium in the Baltic Sea determined by shipboard HPLC analysis. Limnol Oceanogr 27: 366-347.Ayukai T, Okaji K and Lucas JS (1997) Food limitation in the growth and development of crown-of-thorns starfish larvae in the Great Barrier Reef. 1: 621-626. In: Proceedings of the 8th International Coral Reef Symposium, Panama.
- Hierarchy level
- Dataset
- Maintenance and update frequency
- As needed
Metadata
- Metadata identifier
- 41bda75c-97d3-48a1-ace3-96159c97e2eb
- Language
- English
- Character encoding
- UTF8
Point of contact
- Hours of service
- 0800 to 1640 UTC+10: Monday to Friday
Type of resource
- Resource scope
- Dataset
- Metadata linkage
-
Point of truth URL of this metadata record
Point of truth URL of this metadata record
- Date info (Creation)
- 2010-11-19T00:00:00
- Date info (Revision)
- 2017-11-20T00:00:00
Metadata standard
- Title
- ISO 19115-3:2018
Overviews
Spatial extent
Provided by
