Sterol and fatty acid compositions of seagrasses from Magnetic Island, North Queensland
Fresh leaves of seven species of tropical seagrasses, collected from Magnetic Island, north Queensland, were analysed for sterol and fatty acid composition. The species examined were Cymodocea serrulata, Enhalus acoroides, Halodule uninervis, Halophila ovalis, Halophila ovata, Halophila spinulosa and Thalassia hemprichii.Samples were extracted with CHC13-MeOH (1:1). Sterols and fatty acid methyl esters were prepared from the extracts. Sterols (as the methyl ethers) and fatty acid methyl esters were analysed by GLC.Fatty acids and sterols were identified by their mass spectra and by co-chromatography with standards where available.
This research was undertaken to determine if chemical markers were present in seagrass leaves, which would allow seagrass derived material to be traced through coastal ecosystems. Variations in sterol and fatty acid composition were examined for potential chemotaxonomic significance.
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- Date (Revision)
- 2024-10-17T00:00:00
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- AIMS Web Site
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- AIMS Web Site
- Credit
- Gillan, Francis T, Dr (Principal Investigator)
- Status
- Completed
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P1Y0M0DT0H0M0S
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- Oceans
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- Region 1
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- 1982-01-01
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- 1984-06-01
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- Not planned
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- Physical measurement
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The sterol and fatty acid compositions of seven tropical seagrasses from North Queensland, Australia: Gillan FT (1984) The sterol and fatty acid compositions of seven tropical seagrasses from North Queensland, Australia. Phytochemistry 23: 2817-2821.
The sterol and fatty acid compositions of seven tropical seagrasses from North Queensland, Australia: Gillan FT (1984) The sterol and fatty acid compositions of seven tropical seagrasses from North Queensland, Australia. Phytochemistry 23: 2817-2821.
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- Statement
- Statement: Samples were extracted with CHC13-MeOH (1:1) as described in:Hogg RW and Gillan FT (1984) Phytochemistry 23, 93.Sterols and fatty acid methyl esters were prepared from the extracts by the procedures described in:Hogg RW and Gillan FT (1984) Phytochemistry 23, 93.Gillan FT, McFadden GI, Wetherbee R and Johns RB (1981) Phytochemistry 20, 1935.Gillan FT (1983) J. Chromatogr. Sci. 21, 293.Sterols (as the methyl ethers) and fatty acid methyl esters were analysed by GLC using a fused silica SP2100 column (50m x 0.2mm i.d., Hewlett-Packard) and fatty acid esters were additionally analysed on a polar Superox 0.1 fused silica capillary column (50 m x 0.2 mm i.d., SGE Australia).GC/MS analyses were performed on a fused silica cross-linked methyl silicone column (50 m x 0.2 mm i.d., 0.11 µm phase thickness, ULTRA#1, Hewlett-Packard) with the HP5970A mass selective detector directly coupled to the gas chromatograph. Ionization voltage was 70 eV. Spectra were scanned repetitively at 600 amu/sec.Fatty acids and sterols were identified by their mass spectra and by co-chromatography with standards where available. Authentic standards of sterols were provided by the Medical Research Council (UK).
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- 22251eaf-51b6-4e00-9a4a-8cfeb3564320
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- English
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- UTF8
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- Date info (Creation)
- 2010-04-27T00:00:00
- Date info (Revision)
- 2023-04-20T00:00:00
Metadata standard
- Title
- ISO 19115-3:2018