AIMS Geonetwork
Extracellular phosphatase enzyme activity in reef waters of the central Great Barrier Reef
A fluorometric procedure was applied to measure changes in phosphatase activity in seawater flowing across reef flats at Bowl and Hopkinson Reefs on the central Great Barrier Reef. Seawater samples were collected at 50 m intervals across the reef flat during low tide, commencing at the reef front using a volume transport method and a time-distance-depth record was maintained allowing measurements to relate to the distance a water mass had flowed across the coral reef flat. Water samples were collected below the surface using a dip-scoop, filtered through 10 µm mesh and stored in the dark before being assayed.Fluorescence was measured on 2 ml filtered (Nucleopore, 0.2 µm) and unfiltered subsamples on addition of 50 µl of a 5.0 mM solution of the enzyme substrate (4-methylumbelliferyl phosphate) and then at hourly intervals. An 8 hour period of incubation was required to determine statistically significant rates of hydrolysis from oligotrophic water samples.
This research was initiated to examine the importance of the hydrolysis of organophosphates by alkaline phosphatase enzymes as a source of inorganic phosphate, a nutrient essential for primary production. The assay used was able to determine low levels of enzyme phosphatase activity in marine waters based on the enzymatic hydrolysis of the fluorogenic substrate, 4-methylumbelliferyl phosphate.
Simple
Identification info
- Date (Revision)
- 2024-10-17T00:00:00
Publisher
Owner
- Website
- AIMS Web Site
- Website
- AIMS Web Site
- Credit
- Dunlap, Walter C, Dr (Principal Investigator)
- Status
- Completed
Principal investigator
Point of contact
- Temporal resolution
-
P1Y0M0DT0H0M0S
- Topic category
-
- Oceans
Extent
- Description
- Hopkinson Reef
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Extent
- Description
- Bowl Reef
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Extent
- Description
- Collective resources start and end dates
Temporal extent
- Time position
- 1984-05-01
- Time position
- 1984-05-31
- Maintenance and update frequency
- Not planned
Resource constraints
- Linkage
-
http://i.creativecommons.org/l/by-nc/3.0/au/88x31.png
License Graphic
- Title
- Creative Commons Attribution-NonCommercial 3.0 Australia License
- Website
-
http://creativecommons.org/licenses/by-nc/3.0/au/
License Text
- Other constraints
- Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.
- Other constraints
- Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). Extracellular phosphatase enzyme activity in reef waters of the central Great Barrier Reef. https://apps.aims.gov.au/metadata/view/0b8da168-20b7-4cce-95e9-4071dfc2c354, accessed[date-of-access]".
- Other constraints
- Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.
- Language
- English
- Character encoding
- UTF8
Content Information
- Content type
- Physical measurement
Distribution Information
Distributor
Distributor
- OnLine resource
-
Measurement of extra-cellular phosphatase enzyme activity in reef waters of the central Great Barrier Reef, Australia: Dunlap WC (1985) Measurement of extra-cellular phosphatase enzyme activity in reef waters of the central Great Barrier Reef, Australia. 3: 451-456. In: Proceedings of the 5th International Coral Reef Congress, Tahiti, 27 May-1 June 1985. Antenne Museum-Ephe.
Measurement of extra-cellular phosphatase enzyme activity in reef waters of the central Great Barrier Reef, Australia: Dunlap WC (1985) Measurement of extra-cellular phosphatase enzyme activity in reef waters of the central Great Barrier Reef, Australia. 3: 451-456. In: Proceedings of the 5th International Coral Reef Congress, Tahiti, 27 May-1 June 1985. Antenne Museum-Ephe.
Resource lineage
- Statement
- Statement: The volume transport method used in collection of water samples is described in:Barnes DJ (1983) Profiling coral reef productivity and calcification using pH and oxygen electrodes. J. Exp. Mar. Biol. Ecol. 66: 149-169.Fluorescence was measured with a Turner spectrofluorometer, Model 430. Excitation wavelength was 364 nm (15 nm bandwidth) with a 7-60 band-pass filter (315-385 nm) to remove stray light artifacts. The emission wavelength was 458 nm (15 nm bandwidth) and a secondary 418 nm short wavelength cut-off filter was used to assist resolution of the 4-methylumbelliferone fluorescence from that of the unhydrolyzed enzyme substrate. Standard calibration was performed with 4- methylumbelliferone (Sigma Chemical) in filtered sterilized seawater (0.2 um). The calibration curve was linear between the intensity of 4- methylumbelliferone fluorescence and its concentration in the range 5 x 10-9 M to 2 x 10-6 M.Phosphatase enzyme activities were calculated using linear regression analyses and standard error determination provided values to calculate 95% confidence limits. Phosphatase enzyme activities are expressed in units of pmol substrate (or PO4) hydrolyzed/ml/h. Heat denatured sterile seawater (1 h at 100°C) was used for reagent controls. Blank values due to spontaneous, non-enzymatic hydrolysis of the substrate were substracted from the fluorescence assay as background (0.25 pmol substrate hydrolyzed/ml/h or lower). Spontaneous hydrolysis of the enzyme substrate was minimized by thorough cleaning of all glassware used in the analysis. Phosphate based detergents must be avoided as organophosphates are competitive substrates and orthophosphate inhibits the enzyme activity.
- Hierarchy level
- Dataset
- Maintenance and update frequency
- As needed
Metadata
- Metadata identifier
- 0b8da168-20b7-4cce-95e9-4071dfc2c354
- Language
- English
- Character encoding
- UTF8
Point of contact
- Hours of service
- 0800 to 1640 UTC+10: Monday to Friday
Type of resource
- Resource scope
- Dataset
- Metadata linkage
-
Point of truth URL of this metadata record
Point of truth URL of this metadata record
- Date info (Creation)
- 2009-11-24T00:00:00
- Date info (Revision)
- 2018-04-30T00:00:00
Metadata standard
- Title
- ISO 19115-3:2018
Overviews
Spatial extent
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